mtt working solution  (Macklin Inc)

Journal: Frontiers in Pediatrics

Article Title: Acellular foreskin dermal matrix is efficient in supporting the growth of urothelial cells derived from hypospadias patients

doi: 10.3389/fped.2025.1628435

Figure Lengend Snippet: Evaluation of cytotoxicity of foreskin ADM on primary urothelium-derived cells. MTT assay results showing the viability of primary urothelium-derived cells cultured in media conditioned with various concentrations of foreskin ADM soaking solution for 24 or 48 h. One-way ANOVA was used to assess statistical significance. “ns” denotes no significant difference.

Article Snippet: Cells were seeded into 96-well plates at a density of 1 × 104 cells per well (200 μl) and cultured for 48 h. Afterward, 10 μl MTT working solution (5 mg/ml, Yeasen) was added to each well, and the plates were incubated at 37°C for 4 h. Then, 150 μl DMSO was added to each well, followed by 10 min of agitation at 37°C.

Techniques: Derivative Assay, MTT Assay, Cell Culture